Journal:

Article Title: In Vitro Antibacterial Properties of Pexiganan, an Analog of Magainin

doi:

Figure Lengend Snippet: Distribution of MICs of pexiganan for aerobic bacteria

Article Snippet: The MIC of pexiganan was >256 μg/ml for Proteus mirabilis and Serratia marcescens , as noted previously for magainins ( 26 ). table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 ATCC strain MIC (μg/ml) Pexiganan MSI-214 Staphylococcus aureus ATCC 29213 8–16 16 Staphylococcus aureus ATCC 33591 (methicillin resistant) 16–32 ND a Staphylococcus epidermidis ATCC 12228 2–4 ND Enterococcus faecium ATCC 51559 (vancomycin resistant) 8 4 Enterococcus faecalis ATCC 29212 64 16 Group A Streptococcus ATCC 49399 4 ND Streptococcus pneumoniae ATCC 49619 32 ND Streptococcus bovis ATCC 49133 4 4 Bacillus cereus ATCC 11778 8 ND Micrococcus luteus ATCC 4698 2 2–4 Corynebacterium group A ATCC 49676 2–4 ND Escherichia coli ATCC 25922 8–16 8–16 Enterobacter cloacae ATCC 35030 8 ND Proteus mirabilis ATCC 29245 >256 >256 Serratia marcescens ATCC 8100 >256 >256 Helicobacter pylori ATCC 43504 2 ND Haemophilus influenzae ATCC 49247 8 ND Pseudomonas aeruginosa ATCC 27853 8–16 16–32 Bacteroides fragilis ATCC 90028 2–4 ND Bacteroides thetaiotaomicron ATCC 29741 2–4 ND Clostridium difficile ATCC 43255 4 ND Propionibacterium acnes ATCC 29399 8 ND Veillonella parvula ATCC 10790 8 ND Open in a separate window a ND, the MIC was not determined.

Techniques:

Journal:

Article Title: In Vitro Antibacterial Properties of Pexiganan, an Analog of Magainin

doi:

Figure Lengend Snippet: MICs of pexiganan and all- d -amino-acid pexiganan (MSI-214) for ATCC reference strains

Article Snippet: The MIC of pexiganan was >256 μg/ml for Proteus mirabilis and Serratia marcescens , as noted previously for magainins ( 26 ). table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 ATCC strain MIC (μg/ml) Pexiganan MSI-214 Staphylococcus aureus ATCC 29213 8–16 16 Staphylococcus aureus ATCC 33591 (methicillin resistant) 16–32 ND a Staphylococcus epidermidis ATCC 12228 2–4 ND Enterococcus faecium ATCC 51559 (vancomycin resistant) 8 4 Enterococcus faecalis ATCC 29212 64 16 Group A Streptococcus ATCC 49399 4 ND Streptococcus pneumoniae ATCC 49619 32 ND Streptococcus bovis ATCC 49133 4 4 Bacillus cereus ATCC 11778 8 ND Micrococcus luteus ATCC 4698 2 2–4 Corynebacterium group A ATCC 49676 2–4 ND Escherichia coli ATCC 25922 8–16 8–16 Enterobacter cloacae ATCC 35030 8 ND Proteus mirabilis ATCC 29245 >256 >256 Serratia marcescens ATCC 8100 >256 >256 Helicobacter pylori ATCC 43504 2 ND Haemophilus influenzae ATCC 49247 8 ND Pseudomonas aeruginosa ATCC 27853 8–16 16–32 Bacteroides fragilis ATCC 90028 2–4 ND Bacteroides thetaiotaomicron ATCC 29741 2–4 ND Clostridium difficile ATCC 43255 4 ND Propionibacterium acnes ATCC 29399 8 ND Veillonella parvula ATCC 10790 8 ND Open in a separate window a ND, the MIC was not determined.

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Journal:

Article Title: In Vitro Antibacterial Properties of Pexiganan, an Analog of Magainin

doi:

Figure Lengend Snippet: Effect of pH on MIC of pexiganan when tested in nutrient broth

Article Snippet: The MIC of pexiganan was >256 μg/ml for Proteus mirabilis and Serratia marcescens , as noted previously for magainins ( 26 ). table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 ATCC strain MIC (μg/ml) Pexiganan MSI-214 Staphylococcus aureus ATCC 29213 8–16 16 Staphylococcus aureus ATCC 33591 (methicillin resistant) 16–32 ND a Staphylococcus epidermidis ATCC 12228 2–4 ND Enterococcus faecium ATCC 51559 (vancomycin resistant) 8 4 Enterococcus faecalis ATCC 29212 64 16 Group A Streptococcus ATCC 49399 4 ND Streptococcus pneumoniae ATCC 49619 32 ND Streptococcus bovis ATCC 49133 4 4 Bacillus cereus ATCC 11778 8 ND Micrococcus luteus ATCC 4698 2 2–4 Corynebacterium group A ATCC 49676 2–4 ND Escherichia coli ATCC 25922 8–16 8–16 Enterobacter cloacae ATCC 35030 8 ND Proteus mirabilis ATCC 29245 >256 >256 Serratia marcescens ATCC 8100 >256 >256 Helicobacter pylori ATCC 43504 2 ND Haemophilus influenzae ATCC 49247 8 ND Pseudomonas aeruginosa ATCC 27853 8–16 16–32 Bacteroides fragilis ATCC 90028 2–4 ND Bacteroides thetaiotaomicron ATCC 29741 2–4 ND Clostridium difficile ATCC 43255 4 ND Propionibacterium acnes ATCC 29399 8 ND Veillonella parvula ATCC 10790 8 ND Open in a separate window a ND, the MIC was not determined.

Techniques:

Journal:

Article Title: In Vitro Antibacterial Properties of Pexiganan, an Analog of Magainin

doi:

Figure Lengend Snippet: Effect of sera from different animals on antibacterial activity of pexiganan a

Article Snippet: The MIC of pexiganan was >256 μg/ml for Proteus mirabilis and Serratia marcescens , as noted previously for magainins ( 26 ). table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 ATCC strain MIC (μg/ml) Pexiganan MSI-214 Staphylococcus aureus ATCC 29213 8–16 16 Staphylococcus aureus ATCC 33591 (methicillin resistant) 16–32 ND a Staphylococcus epidermidis ATCC 12228 2–4 ND Enterococcus faecium ATCC 51559 (vancomycin resistant) 8 4 Enterococcus faecalis ATCC 29212 64 16 Group A Streptococcus ATCC 49399 4 ND Streptococcus pneumoniae ATCC 49619 32 ND Streptococcus bovis ATCC 49133 4 4 Bacillus cereus ATCC 11778 8 ND Micrococcus luteus ATCC 4698 2 2–4 Corynebacterium group A ATCC 49676 2–4 ND Escherichia coli ATCC 25922 8–16 8–16 Enterobacter cloacae ATCC 35030 8 ND Proteus mirabilis ATCC 29245 >256 >256 Serratia marcescens ATCC 8100 >256 >256 Helicobacter pylori ATCC 43504 2 ND Haemophilus influenzae ATCC 49247 8 ND Pseudomonas aeruginosa ATCC 27853 8–16 16–32 Bacteroides fragilis ATCC 90028 2–4 ND Bacteroides thetaiotaomicron ATCC 29741 2–4 ND Clostridium difficile ATCC 43255 4 ND Propionibacterium acnes ATCC 29399 8 ND Veillonella parvula ATCC 10790 8 ND Open in a separate window a ND, the MIC was not determined.

Techniques: Activity Assay

Journal:

Article Title: In Vitro Antibacterial Properties of Pexiganan, an Analog of Magainin

doi:

Figure Lengend Snippet: Killing curve study. The killing activity of pexiganan against four ATCC strains was monitored for the first 2 h. (a) Escherichia coli ATCC 25922; (b) Enterococcus faecium ATCC 51559 (vancomycin resistant); (c) Staphylococcus aureus ATCC 29213; (d) Pseudomonas aeruginosa ATCC 27853. For P. aeruginosa, the killing curves were identical (overlapping in the figure) for 4× the MIC and 16× the MIC. Under the assay conditions used in this study, the lowest detectable level was 5 CFU/ml.

Article Snippet: The MIC of pexiganan was >256 μg/ml for Proteus mirabilis and Serratia marcescens , as noted previously for magainins ( 26 ). table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 ATCC strain MIC (μg/ml) Pexiganan MSI-214 Staphylococcus aureus ATCC 29213 8–16 16 Staphylococcus aureus ATCC 33591 (methicillin resistant) 16–32 ND a Staphylococcus epidermidis ATCC 12228 2–4 ND Enterococcus faecium ATCC 51559 (vancomycin resistant) 8 4 Enterococcus faecalis ATCC 29212 64 16 Group A Streptococcus ATCC 49399 4 ND Streptococcus pneumoniae ATCC 49619 32 ND Streptococcus bovis ATCC 49133 4 4 Bacillus cereus ATCC 11778 8 ND Micrococcus luteus ATCC 4698 2 2–4 Corynebacterium group A ATCC 49676 2–4 ND Escherichia coli ATCC 25922 8–16 8–16 Enterobacter cloacae ATCC 35030 8 ND Proteus mirabilis ATCC 29245 >256 >256 Serratia marcescens ATCC 8100 >256 >256 Helicobacter pylori ATCC 43504 2 ND Haemophilus influenzae ATCC 49247 8 ND Pseudomonas aeruginosa ATCC 27853 8–16 16–32 Bacteroides fragilis ATCC 90028 2–4 ND Bacteroides thetaiotaomicron ATCC 29741 2–4 ND Clostridium difficile ATCC 43255 4 ND Propionibacterium acnes ATCC 29399 8 ND Veillonella parvula ATCC 10790 8 ND Open in a separate window a ND, the MIC was not determined.

Techniques: Activity Assay

Journal:

Article Title: In Vitro Antibacterial Properties of Pexiganan, an Analog of Magainin

doi:

Figure Lengend Snippet: Changes in MIC after sequential in vitro passages with a subinhibitory concentration

Article Snippet: The MIC of pexiganan was >256 μg/ml for Proteus mirabilis and Serratia marcescens , as noted previously for magainins ( 26 ). table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 ATCC strain MIC (μg/ml) Pexiganan MSI-214 Staphylococcus aureus ATCC 29213 8–16 16 Staphylococcus aureus ATCC 33591 (methicillin resistant) 16–32 ND a Staphylococcus epidermidis ATCC 12228 2–4 ND Enterococcus faecium ATCC 51559 (vancomycin resistant) 8 4 Enterococcus faecalis ATCC 29212 64 16 Group A Streptococcus ATCC 49399 4 ND Streptococcus pneumoniae ATCC 49619 32 ND Streptococcus bovis ATCC 49133 4 4 Bacillus cereus ATCC 11778 8 ND Micrococcus luteus ATCC 4698 2 2–4 Corynebacterium group A ATCC 49676 2–4 ND Escherichia coli ATCC 25922 8–16 8–16 Enterobacter cloacae ATCC 35030 8 ND Proteus mirabilis ATCC 29245 >256 >256 Serratia marcescens ATCC 8100 >256 >256 Helicobacter pylori ATCC 43504 2 ND Haemophilus influenzae ATCC 49247 8 ND Pseudomonas aeruginosa ATCC 27853 8–16 16–32 Bacteroides fragilis ATCC 90028 2–4 ND Bacteroides thetaiotaomicron ATCC 29741 2–4 ND Clostridium difficile ATCC 43255 4 ND Propionibacterium acnes ATCC 29399 8 ND Veillonella parvula ATCC 10790 8 ND Open in a separate window a ND, the MIC was not determined.

Techniques: In Vitro

Journal:

Article Title: Molecular Epidemiology of Ampicillin-Resistant Non-?-Lactamase-Producing Haemophilus influenzae

doi:

Figure Lengend Snippet: Characteristics of AMP r β− H. influenzae : patterns obtained by different molecular biology techniques a

Article Snippet: Using these different techniques, we spotted several groups of strains, as described Table . table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Strains clustered in the same group according to the results of the following technique a : RT AP-PCR (217δ2) AP-PCR (RapIV) PFGE 1 (ATCC 49247), 7, 15, 19, 24, 25 1 (ATCC 49247), 7, 15, 18, 24, 25 1 (ATCC 49247), 7, 15, 19, 24, 25 1 (ATCC 49247), 7, 24, 25 2 (ATCC 49766), 11 2 (ATCC 49766), 11 11, 14 2 (ATCC 49766), 11 26, 27 26, 27, ±14 (one band difference) 26, 27 26, 27, 14 28, 29 28, 29 28, ±29 (one band difference) 28, 29 4, ±5 (one band difference) 4, ±5 (one band difference), 22, 31 ±4, 5, 23 (one band difference) 4, 5, 22, 23, 31 Open in a separate window a ±, the strain shared only some characteristics with the other strains in the group.

Techniques:

Journal:

Article Title: Molecular Epidemiology of Ampicillin-Resistant Non-?-Lactamase-Producing Haemophilus influenzae

doi:

Figure Lengend Snippet: Patterns for AMPr β− H. influenzae strains obtained by AP-PCR with primer 217δ2. The dendrogram and AP-PCR profiles obtained with primer 217δ2 of H. influenzae were analyzed with Taxotron software. Strains 1 to 31 are described in Table ​Table1.1. The Molecular Weight III marker was from Boehringer Mannheim. S, strains; D, areas.

Article Snippet: Using these different techniques, we spotted several groups of strains, as described Table . table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Strains clustered in the same group according to the results of the following technique a : RT AP-PCR (217δ2) AP-PCR (RapIV) PFGE 1 (ATCC 49247), 7, 15, 19, 24, 25 1 (ATCC 49247), 7, 15, 18, 24, 25 1 (ATCC 49247), 7, 15, 19, 24, 25 1 (ATCC 49247), 7, 24, 25 2 (ATCC 49766), 11 2 (ATCC 49766), 11 11, 14 2 (ATCC 49766), 11 26, 27 26, 27, ±14 (one band difference) 26, 27 26, 27, 14 28, 29 28, 29 28, ±29 (one band difference) 28, 29 4, ±5 (one band difference) 4, ±5 (one band difference), 22, 31 ±4, 5, 23 (one band difference) 4, 5, 22, 23, 31 Open in a separate window a ±, the strain shared only some characteristics with the other strains in the group.

Techniques: Software, Molecular Weight, Marker

Journal:

Article Title: Molecular Epidemiology of Ampicillin-Resistant Non-?-Lactamase-Producing Haemophilus influenzae

doi:

Figure Lengend Snippet: Different strain groups obtained by four molecular biology techniques a

Article Snippet: Using these different techniques, we spotted several groups of strains, as described Table . table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Strains clustered in the same group according to the results of the following technique a : RT AP-PCR (217δ2) AP-PCR (RapIV) PFGE 1 (ATCC 49247), 7, 15, 19, 24, 25 1 (ATCC 49247), 7, 15, 18, 24, 25 1 (ATCC 49247), 7, 15, 19, 24, 25 1 (ATCC 49247), 7, 24, 25 2 (ATCC 49766), 11 2 (ATCC 49766), 11 11, 14 2 (ATCC 49766), 11 26, 27 26, 27, ±14 (one band difference) 26, 27 26, 27, 14 28, 29 28, 29 28, ±29 (one band difference) 28, 29 4, ±5 (one band difference) 4, ±5 (one band difference), 22, 31 ±4, 5, 23 (one band difference) 4, 5, 22, 23, 31 Open in a separate window a ±, the strain shared only some characteristics with the other strains in the group.

Techniques: